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Top Clinical Insights: ANA Q&A (Antinuclear Antibodies)

  • It is NOT recommended to order an ANA for patients without signs/symptoms/history suggestive of a specific rheumatic skin disease or if you do not have high suspicion for rheumatic skin disease.

  • ANA detection by IFA (indirect immunofluorescence assay) is the gold standard.

  • If ANA IFA positive in a patient with signs/symptoms of rheumatic disease, then order disease specific antibodies (ENA) testing.